"Purpose: This study was done to develop the self-report Mother-Preschool Child Interaction Scale (MPIS) for mothers of preschool children. Methods: The scale was based on items derived from literature review and in-depth interviews. A methodological study was used to check reliability and validity and participants were 334 mothers of preschool children enrolled in kindergarten or nursery. Data were analyzed using principal component factor analysis for construct validity, t-test for contrasted group validity, Pearson correlation for criterion related validity and test-retest reliability and Cronbach’s α for reliability. Results: In the final MPIS 34 items identified through factor analysis were included, 6 constructs were derived, and explanatory power was 64.2%. Items on the MPIS were verified through correlation with the interaction observation scale of Kim qqqamp; Mahoney and MPIS. Results were significant as mothers in the normal group exhibited MPIS scores that were significantly higher than those of mothers in the depressed group. Reliability of MPIS was .96 and test-retest reliability was .92. Conclusion: MPIS has the advantage of being easy to use, economical, and useful. Consequently, it is expected to be used as a screening tool for promptly and simply identifying the mother-preschool child interaction in diverse nursing practice and research."