Objectives: The study aimed to determine the prevalence of and the relationship between stress and musculoskeletal symptoms among undergraduate students. Methods: The convenience sampling method was used for this cross-sectional survey that was conducted from June 16, 2014 to June 21, 2014. A total of 223 subjects (88 men, 135 women) participated in the study and completed a comprehensive self-administered questionnaire requesting information on demographics, medical history, lifestyle, and musculoskeletal symptoms. The Depression Anxiety Stress Scale (DASS) was also administered to the subjects. A total of 223 subjects from 12 universities completed the questionnaires; the data obtained was used for the final statistical analysis. Results: Women had a higher prevalence of stress and musculoskeletal symptoms. Adjusting for sex and age, subjects who were stressed had a 2.85-fold higher risk of musculoskeletal symptoms than subjects who were not stressed. Stratified analyses by sex also showed that women had a higher risk of musculoskeletal symptoms (Modified OR 2.98, 95% CI 1.38-6.45). Conclusions: We found a positive association between stress and musculoskeletal symptoms in undergraduate students; sex differences were present. Further dose-response relationship between stress and musculoskeletal symptoms will be identified by a longitudinal study with a larger sample size.