The purpose of this study is to examine the influence of the organizational characteristic and workers characteristics of Family Business Organization on its Business Performance. The subjects of this study were workers of healthy family support centers and multicultural family support centers. A survey was conducted from July 30th to September 30th. A total 372 materials were used for analysis of final data and SPSS(Statistical Package for the Social Sciences) WIN 18.0 program was employed in analyzing the collected data. As the result of analysing the effect of organizational characteristics and culture and characteristics of workers on business performance through hierarchical regression analysis, higher performance was achieved when the agency's organizational culture has the characteristics of a developed culture, and when the operating body is a school principal corporation rather than a non-profit corporation, and in the city rather than farming town, having enough information and external resources at the center, and when the job satisfaction of workers are high, and when organizational culture is more hierarchical. There were difference in the result of analysing the effect of organizational characteristic and culture and characteristic of workers on business performance. The level of service and business value of Family Service Organization was higher when the agency's organizational culture has the characteristics of a developed culture, hierarchical culture and agreement culture, and when operating body is a school principal corporation rather than a non-profit corporation, having enough information and external resources at the center, and the administrative skills of workers are higher. Business volume is increased when the operating body is a school principal corporation rather than a non-profit corporation, and in the city rather than farming town, and when the agency's organizational culture has the characteristics of hierarchical culture and agreement culture, having enough external resources at the center, and the practicing ability of workers are higher. When it comes to business efficiency, it is increased when the agency's organizational culture has the characteristics of developed culture and the job satisfaction of workers are high, having enough external and financial resources at the center. Therefore we will need to enhance business performance through improved management and job satisfaction of employees in organizational culture.