본 연구는 일-가족 양립갈등, 조직문화, 상사의 지지 요인들이 정신적, 신체적 건강과 어떤 관계가 있는지 살펴보았다. 본 연구를 위해 한 종합병원에서 근무하는 200명의 기혼간호사들을 대상으로 2013년 4월 10일부터 4월 16일까지 설문조사를 실시하였다. 연구결과를 보면, 첫째, 가족갈등이 높을수록 정신적 건강과 신체적 건강에 부정적인 영향을 끼치는 것으로 나타났다. 둘째, 직장갈등은 정신적 건강에 부정적인 영향을 미치는 것으로 나타났으나, 신체적 건강에는 영향을 미치지 못한 것으로 나타났다. 셋째, 상사의 지지가 높을수록 정신적 건강과 신체적 건강에 긍정적인 영향을 끼치는 것으로 나타났다. 본 연구를 통해 기혼간호사들의 일과 가족의 갈등을 감소시키고 정신적·신체적 건강을 높일 수 있는 조직 내 상사의 지지가 중요하다는 것을 알 수 있었다. 본 연구는 간호사들의 일-가족 양립갈등을 감소시키기 위한 함의를 제공한다.;The purpose of this study was to investigate the effects of work-family conflicts, organizational culture, and the support of supervisors that could influence the work-family conflicts of married nurses on their mental and physical health. The study took a survey with 200 married nurses working at a general hospital from April 10 to 16, 2013. The research findings were as follows: First, the more family conflicts married nurses had, the poorer mental and physical health they had. Second, workplace conflicts had impacts on their mental health but no impacts on their physical health. Third, the more support they received from their supervisors, the more positive effects it had on their mental and physical health. Those findings indicate that a high level of supervisor support at the workplace reduces work-family conflicts and improves married nurses’ mental and physical health. The study found that the more work-family conflicts married nurses had, the worse effects they had on their mental and physical health and that supervisor support was important for helping to reduce work and family conflicts among married nurses and improve their mental and physical health. Based on those findings, the study proposed effective alternatives and improvement measures to help to reduce their work-family conflicts.