Purpose: The purpose of this study was to evaluate the effects of essential oil on oxidative stress, immunity, and skin condition inatopic dermatitis (AD) induced mice. Methods: This study was a 3×3 factorial design. Factors were oil type (Lavender, Thyme, and 2:1mixture of lavender and thyme oil [blending oil]) and treatment period (0 day, 7 days, and 21 days). The samples were 45 mice with ADand randomly assigned to nine groups of five mice per group. The dependent variables such as superoxide radical, IgE, degranulatedmast cells, and epidermal thickness were measured. Data were collected from February to April in 2014. Descriptive statistics, One-wayANOVA, Two-way ANOVA, and Tukey’s HSD test were performed using the SPSS WIN 20.0 program. Results: Dependent variables werenot statistically significantly different by the three oil types (pqqqgt;.05). Essential oils such as lavender, thyme, and blending oil were all effectivein reducing AD symptoms and especially 2:1 blending oil were most effective. There were statistically significant differences bythe three treatment periods in all dependent variables (pqqqlt;.001). There were statistically significant interactions between oil types andtreatment periods in all dependent variables (pqqqlt;.01). For decreasing superoxide radical, degranulated mast cells, and epidermal thickness,2:1 mixed oil should be applied for at least 21 days. Otherwise to reduce IgE, 2:1 mixed oil should be used for at least 7 days. Conclusion: These findings provide bases for developing effective interventions for AD patients to manage their AD symptoms.