Purpose: The purpose of this cross-over experimental study was to examine effects of music intervention on maternal anxiety, fetal heartrate pattern and testing time during non-stress tests (NST) for antenatal fetal assessment. Methods: Sixty pregnant women within 28 to 40gestational weeks were randomly assigned to either the experimental group (n=30) or control group (n=30). Music intervention was providedto pregnant women in the experimental group during NST. Degree of maternal anxiety and fetal heart rate pattern were our primaryoutcomes. State-trait anxiety inventory, blood pressure, pulse rate, and changes in peripheral skin temperature were assessed to determinethe degree of maternal anxiety. Baseline fetal heart rate, frequency of acceleration in fetal heart rate, fetal movement test and testingtime for reactive NST were assessed to measure the fetal heart rate pattern Results: The experimental group showed significantly lowerscores in state anxiety than the control group. There were no significant differences in systolic blood pressure and pulse rate betweenthe two groups. Baseline fetal heart rate was significantly lower in the experimental group than in the control group. Frequency of accelerationin fetal heart rate was significantly increased in the experimental group compared to the control group. There were no significantdifferences in fetal movement and testing time for reactive NST between the two groups. Conclusion: Present results suggest that musicintervention could be an effective nursing intervention for alel viating anxiety during non-stress test.