Purpose: This aim of this phenomenological study was to describe and understand the experience of spiritual conflict in hospice nurses by identifyingthe meanings and structures of the experience. Methods: Participants were 12 nurses working for one year or more at hospice units ofgeneral hospitals in a metropolitan city and experiencing of spiritual conflict as hospice nurses. Over six months data were collected using individualin-depth interviews and analyzed with the method suggested by Colaizzi. Results: The experience of spiritual conflict in participants wasorganized into three categories, six theme-clusters, and 13 themes. The participants felt existential anxiety on death and a fear of death which isout of human control and skepticism for real facts of human beings facing death. They also experienced agitation of fundamental beliefs aboutlife with agitation of the philosophy of life guiding themselves and mental distress due to fundamental questions that are difficult to answer. Alsothey had distress about poor spiritual care with guilty feelings from neglecting patients’spiritual needs and difficulties in spiritual care due to lackof practical competencies. Conclusion: Findings indicate the experience of spiritual conflict in hospice nurses is mainly associated with frequentexperience of death in hospice patients. The experience of spiritual conflict consisted of existential anxiety, agitation of fundamental beliefs anddistress over poor spiritual care. So, programs to help relieve anxiety, agitation and distress are necessary to prevent spiritual conflict and thenspiritual burnout in hospice nurses.