Objectives: The perceptions and attitudes of hospital staff to smoking directly affect patients and other staff. It is important to improve knowledge of smoking more than any other occupation to improve the health of the whole user and to fulfill the responsibility of the medical professional. Methods: This study was conducted by a self-reported survey from August to September 2015. 652(59%) out of about 1,100 staff participated. Statistical analysis methods used are Chi square tests, t-test, ANOVA, Multiple regression analysis. The statistical program used SAS ver 9.4. Results: About 64% of hospital were smokers and 31% were currently smoking. 76% of people in the hospital experienced secondhand smoke. The scores of hospital workers’ smoking knowledge scores showed an average score of 74.4 on a scale of 100, and both smokers and non-smokers predicted smoking cessation. The factors influencing smoking knowledge were sex, marital status, having a smoker in family, and the factors influencing smoking attitudes were sex, drinking, exercise. Conclusions: This study investigated the smoking-related factors of hospital staff to establish healthy hospital environment. Most respondents respond negatively to their smoking behavior, and specific efforts will be needed to improve awareness of all employees’ smoking cessation.