The purpose of this study was to examine the relationship between Toddlers’negative emotionality and mothers’ parenting stress and the moderating roles of a husbands’cooperation, mother-teacher partnership, and other social support. The participants of this study were 248 mothers with toddlers aged 24-35 months. The method of research used was the questionnaire method. The collected data were mainly analyzed by descriptive statistics, t-test, one-way ANOVA, Scheffé test, Pearson’s correlation and multiple regression analysis. The main results of this study were as follows. First, there were differences in mother’s parenting stress according to individual characteristics such as mothers employment status, parenting costs, mother’s feeling of pregnancy. Second, a moderator effect of a husbands’ cooperation was found in the relationship between toddlers’negative emotionality and mothers’ parenting stress. Therefore, it was confirmed that husband's cooperation is more of an important factor in nursing children with negative emotions than other support systems.