This study examined the effects of organizational culture on staff members' use of management information systems (N = 142) within homeless service organizations (N = 24), using a multilevel model. The Organizational Social Context Questionnaire was used to measure organizational culture, defined by three sub-constructs: (1) proficiency, (2) rigidity, and (3) resistance. Results showed that organizational effects were moderated by gender, with male staff members reporting increased use of the technology in organizations with higher proficiency levels. Female staff members' use was not affected by organizational culture. The study also found substantial variability in organizational culture among homeless service providers. Some scored low on rigidity and resistance but high on proficiency, whereas others scored high on proficiency but low on rigidity and resistance. Overall, the sample showed higher proficiency, resistance, and rigidity compared with a national sample of children's mental providers. Implications of these findings are discussed for implementation and evaluation of new technologies in homeless services.